Journal: Molecules (Basel, Switzerland)

Article Title: Extract from Black Soybean Cultivar A63 Extract Ameliorates Atopic Dermatitis-like Skin Inflammation in an Oxazolone-Induced Murine Model.

doi: 10.3390/molecules27092751

Figure Lengend Snippet: Figure 6. A63 extract prevents eosinophil infiltration toward the inflammatory site via CCL26 expression downregulation and the inactivation of JAK1/STAT6 signaling. qPCR analysis of CCL26 expression in IL-4/IL-13-induced HS68 cells (A). Western blot results of IL-4/IL-13-induced HS68 cells (B). Results are expressed as mean ± standard deviation (### p < 0.001 versus control group, *** p < 0.001 versus IL-4/13 treated group).

Article Snippet: The membranes were blocked with a 5% BSA solution and treated with primary antibodies for anti-phospho-p44/42 MAPK (ERK1/2), phospho-p38 MAPK, phospho-SAPK/JNK, phospho-STAT3, phospho-STAT6, phospho-JAK1, p44/42 MAPK (ERK1/2), p38 MAPK, JNK, STAT3, STAT6, JAK1, and GAPDH (Cell Signaling Technology, MA, USA, 1:1000).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: Infection and Immunity

Article Title: Thioredoxin from Brugia malayi: Defining a 16-Kilodalton Class of Thioredoxins from Nematodes

doi: 10.1128/IAI.71.7.4119-4126.2003

Figure Lengend Snippet: Bm-TRX-1 inhibits activation of p38 MAP kinase. The WCPPCR and WCPQCR alleles of Bm-trx-1 were cloned separately into a eukaryotic expression plasmid vector, and the plasmids were introduced into TPA-differentiated ML-1 cells. The transfected ML-1 cells were stimulated with TNF-α (100 IU/well) for 5 min, and the phosphorylation status of p38 MAP kinase was analyzed on immunostained Western blots of cell extracts. Controls included cells maintained in medium (medium control), cells exposed to the transfection regent (transfection control), and cells transfected with a plasmid containing no insert (plasmid control).

Article Snippet: In preliminary experiments, TPA-differentiated ML-1 cells were stimulated with TNF-α (100 IU/well) for 5, 10, and 30 min, and the phosphorylation status of p38 MAP kinase was analyzed on immunostained Western blots of cell extracts with phosphoPlus p38 MAP kinase antibody kit (Cell Signaling Technology, Beverly, Mass.).

Techniques: Activation Assay, Clone Assay, Expressing, Plasmid Preparation, Transfection, Western Blot